A Practical Guide to Protein Engineering

A Practical Guide to Protein Engineering

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This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a career as a protein engineer. With protein engineering being a fundamental technique in almost all molecular biology labs, the book targets advanced undergraduates and graduate students working in molecular biology, biotechnology and related scientific fields.




Literature Search
Sequence Analysis
Structural Analysis
Protein Expression Hosts and Expression Plasmids
Gene Cloning
Protein Expression
Assay
Gene Mutagenesis
High-Throughput Screening (HTS)
Protein Variants Analysis and Characterization
Continuing from Protein Variants
Employability.


ISBN 978-3-030-56897-9
Article number 9783030568979
Media type Book
Edition number 1st ed. 2020
Copyright year 2020
Publisher Springer, Berlin
Length XV, 202 pages
Illustrations XV, 202 p. 91 illus., 68 illus. in color.
Language English