Basic Cloning Procedures
Basic Cloning Procedures
This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.
3 RNA In Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase
4 PCR-Based Site-Specific Mutagenesis
5 Analysis of Specific Protein-DNA Interactions
6 Gel Electrophoresis, Transfer and Hybridization of Nucleic Acids
7 Immunological Methods for Analysis of Recombinant Proteins
8 Protein Synthesis in Cell-Free Systems.
1 cDNA Synthesis and Cloning
2 DNA Sequencing3 RNA In Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase
4 PCR-Based Site-Specific Mutagenesis
5 Analysis of Specific Protein-DNA Interactions
6 Gel Electrophoresis, Transfer and Hybridization of Nucleic Acids
7 Immunological Methods for Analysis of Recombinant Proteins
8 Protein Synthesis in Cell-Free Systems.
Berzins, Valdis
| ISBN | 978-3-642-48977-8 |
|---|---|
| Article number | 9783642489778 |
| Media type | Book |
| Copyright year | 2012 |
| Publisher | Springer, Berlin |
| Length | VII, 169 pages |
| Illustrations | VII, 169 p. |
| Language | English |
