Described here are the practical applications of flow cytometry in specific biological systems, ranging from cell biology to chromosome analysis and sorting. Three major areas of interest in cell and molecular biology are addressed: - Cell Activation and Biological Response. - Membrane-Ligand Interactions and Cell Identity. - Nuclear Components: Form and Function. Data management, expert systems and cell sorting techniques concerning all aspects of flow cytometry are also presented.

I Cell Activation and Biological Response Modifiers

Cytosolic Events Related to Cell Activation: Potential Interaction Between Signal and Energy Transduction Pathways in Smooth Muscle Cells
Studies of Changes in Cytoplasmic pH and Membrane Potential Using Flow Cytometry
Measurement of Enzyme Kinetics by Flow Cytometry
Endocytosis Analysis by Flow Cytometry: Study of the Endocytosis of Fluoresceinylated Neoglycoproteins via Membrane Lectins of Macrophages
Analysis of Intracellular Antigens by Flow Cytometry: Methods for Cell Permeabilization to Antibodies
Expression and Role of Surface Markers of Immunocompetent Cells in Non-lymphoïd Systems
Change in Lipid Composition and in Membrane Fluidity of Human Peripheral Blood Lymphocytes undergoing Blastic Transformation
II: Membrane - Ligand Interactions and Cell Identity
Cellular Proliferation History by Fluorescent Analysis
Identification and Isolation of Bone Marrow Stem Cells
T.Lymphocytes Recognition Molecules in Homing: A Flow Cytometry Study of Lectin-glycoconjugates Interactions
Three- and Four-color Immunofluorescence Analysis by Flow Cytometry
Energy Transfer
Fluid Mechanical Properties of Flow Cytometers and Assessment Cell-cell Adhesion Forces
Use of Biotin-labeled Growth Factors for Receptor Studies
Cytometric Tout Terrain or Bush DNA Cytometry
III: Nuclear Components: Form and Function
Bromodeoxuridine Procedures for Analysis of DNA Synthesis
Flow Cytogenetics: Fundamentals and New Developments
In situ Hybridization
The Molecular Basis of Mammalian Cell Growth Control
Flow Cytometric Analysis of Nuclear Associated Antigens
Chromatin Changes Accompanying Apoptosis Detected by Flow Cytometry
Gene Mapping and PCR Applications with Flow-sorted Chromosomes
Cytometry,Antitumour Drugs and DNA Topoisomerases
Detection of Apoptosis Using Fluorescent In Situ Nick Translation
IV: Data Management Expert Systems and Cell Sorting Techniques
Flow Cytometry Data Analysis. Basic Concepts and Analysis of Mono-dimensional Immunofluorescence Histograms
Analysis of Multiparameter Flow Cytometric Data
Expert Assistance for Flow Immunophenotyping
DNA Content and Cell Cycle Analysis by Flow Cytometry in Clinical Samples: Applications in Cancer
Immunomagnetic Particles for Cell Isolation
Optical Trapping: Instrumentation and Biological Applications.