1;Chapter 1 Introduction;5 1.1;1.1 Antibody Array Technologies and Applications;5 1.2;1.2 Protein Phosphorylation, Localization and Function;7 1.3;1.3 Interaction Proteomics and Pathway Building;8 2;Chapter 2 Technologies;16 2.1;2.1 Materials;16 2.2;2.2 Methods;18 2.3;2.3 Silver Staining in ArrayTube;21 3;Chapter 3 New Cytoplasmic and Nuclear BMP2- Signaling Pattern in U937 Cells;27 3.1;3.1 3.1 Modulation of Phosphorylation and Localization in BMP2- Treated U937 Cells;27 3.2;3.2 BMP2 Signaling in U937 Cells;36 3.3;3.3 BMP2 Signaling Model in U937 Cells;42 4;Chapter 4 New Cytoplasmic and Nuclear BMP2- Signaling in MCF7 Cells;44 4.1;4.1 Modulation of Phosphorylation and Localization in BMP2- Treated MCF7 Cells;44 4.2;4.2 BMP2 Signaling in MCF7 Cells;52 4.3;4.3 BMP2 Signaling Model in MCF7 Cells;54 5;Chapter 5 New Cytoplasmic and Nuclear STI571 Signaling in K562 Cells;56 5.1;5.1 Modulation of Phosphorylation and Localization in STI571- Treated K562 Cells;56 5.2;5.2 STI571 Signaling in K562 Cells;62 5.3;5.3 STI571 Signaling Model in K562 Cells;65 6;Chapter 6 Positive and Negative Co-localized Rela-tionships in Three Treated Cell Lines;67 6.1;6.1 Positive and Negative Co-localized Distributions;67 6.2;6.2 Possible Relationships;87 7;Chapter 7 Cytoplasmic Signaling in Three Treated Cell Lines;91 7.1;7.1 Cytoplasmic Distribution;91 7.2;7.2 Co-cytoplasmic Signaling;96 8;Chapter 8 Proteomic Silencing in Treated Human Cancer Cell Lines;99 8.1;8.1 Proteomic Silence Distributions;99 8.2;8.2 Possible Relationships;102 9;Chapter 9 Signaling Pathway Setting-up Based on Bioinformatics;104 9.1;9.1 Methods;104 9.2;9.2 Results;110 9.3;9.3 Discussion;121 9.4;9.4 Conclusions;122 10;References;123