Thin-layer chromatography (TLC) is widely used particularly in pharmaceutical and food analysis. While there are a number of books on the qualitative identification of chemical substances by TLC, the unique focus here is on quantitative analysis. The authors describe all steps of the analytical procedure, beginning with relevant basics and equipment for quantitative TLC. They go on with sample pretreatment and sample application, development and staining, scanning and finally present the statistical and chemometric data evaluation as well as validation. An important feature is the coverage of effect-directed biological detection methods.

History of Planar Chromatography

The Theoretical Basis of Thin-Layer Chromatography (TLC)
The Stationary Phase in Thin Layer Chromatography (TLC)
The Mobile Phase in Adsorption and Partition Chromatography
Preparing and Applying Samples
Basis for TLC Development Techniques
Specific Staining Reactions
Bioeffective-linked Analysis in Modern HPTLC
Planar Chromatography Detectors
Diffuse Reflectance from TLC Layers
Fluorescence in TLC Layers
Chemometrics in HPTLC
Reciprocal Model
Statistics for Quantitative TLC
Planning an Analysis and Validation in TLC.