This volume is a continuation of two prior books on advanced electron microscope techniques. The purpose of this series has been to provide in depth analyses of methods which are considered to be at the leading edge of electron microscopic research procedures with applications in the biological sciences. The mission of the present volume remains that of a source book for the research practitioner or advanced student, especially one already well versed in basic electron optical methods. It is not meant to provide in troductory material, nor can this modest volume hope to cover the entire spectrum of advanced technology now available in electron microscopy. In the past decade, computers have found their way into many research laboratories thanks to the enormous increase in computing power and stor age available at a modest cost. The ultrastructural area has also benefited from this expansion in a number of ways which will be illustrated in this volume. Half of the contributions discuss technologies that either directly or indirectly make extensive use of computer methods.

Three-Dimensional Reconstruction of Nonperiodic Macromolecular Assemblies from Electron Micrographs

1 Introduction
2 Methods for Obtaining Projection Data
3 Methods of Three-Dimensional Reconstruction
4 Experimental Results
5 Conclusions
References
High Resolution Biological X-Ray Microanalysis of Diffusable Ions
1 Introduction
2 Principles of the Technique
3 Specimen Preparation
4 Quantitation
5 Applications in the Analysis of Diffusable Ions
6 Conclusion
References
Metal Deposition by High-Energy Sputtering for High Magnification Electron Microscopy
1 Introduction
2 Methods
3 Metal Deposition
4 Conclusion
References
Computer Programs for Biological Stereology
1 Introduction
2 Point Counting Programs
3 Digitizing Programs
4 Special Purpose Stereology Programs
5 Concluding Comments
References
A Guide to Fracture Label: Cytochemical Labeling of Freeze-Fractured Cells
1 Introduction
2 Experimental Procedures
3 Electron Microscopy
4 Interpretation
References
The Preparation of Colloidal Gold Probes and Their Use as Marker in Electron Microscopy
1 Introduction
2 The Preparation and Storage of Colloidal Gold Sols
3 The Production, Purification, and Storage of Gold Probes
4 Quality Control and Analysis of Gold Sols and Probes
5 Critical Evaluation of the Use of Gold Probes in Selected Marking Techniques
6 Conclusions
References.