This textbook provides step-by-step protocols and detailed explanations for RNA Sequencing, ChIP-Sequencing and Epigenetic Sequencing applications.

The reader learns how to perform Next Generation Sequencing data analysis, how to interpret and visualize the data, and acquires knowledge on the statistical background of the used software tools.

Written for biomedical scientists and medical students, this textbook enables the end user to perform and comprehend various Next Generation Sequencing applications and their analytics without prior understanding in bioinformatics or computer sciences.

Chapter 1. Next Generation Sequencing (NGS)- What can be sequenced?

Opportunities and Perspectives of NGS Applications in Cancer Research
Chapter 3.  Library Construction for NGS
Chapter 4. NGS Technologies
Chapter 5. Computer Setup
Chapter 6. Introduction to Command Line
Chapter 7. NGS Data
Chapter 8. Reference Genome
Chapter 9. Alignment
Chapter 10. Identification of Genetic Variants and de novo Mutations Based on NGS
Chapter 11. Design and Analysis of RNA Sequencing Data
Chapter 12. Design and Analysis of Epigenetics and ChIP-Sequencing Data
APPENDIX.