Apply chemical engineering principles to biological processes with??confidence

Biochemical Engineering: A Textbook for Engineers, Chemists, and Biologists, now in its Third Edition, delivers a structured approach to applying chemical engineering principles to biological systems. Written by a team of experienced biochemical engineers from leading Japanese universities, this textbook requires no prior chemical engineering knowledge while building the analytical skills needed to solve real bioprocess problems.

The Third Edition adds a new chapter on monoclonal antibodies and therapeutic proteins, covering upstream processing, downstream purification, and quality control. The three-part structure progresses from fundamental concepts through process aspects like heat transfer, mass transfer, and bioreactors, to practical applications including fermentor engineering and medical device production. More than one hundred exercises support self-study.

The book includes:

• Clear explanations connecting fundamental engineering principles to therapeutic protein production, bridging classical biochemical engineering with modern industrial??biotechnology
• Comprehensive coverage of bioseparation methods, bioreactor design, bioprocess control, and biorecognition assays essential for bioprocess development
• Over one hundred exercises with detailed questions and answers enabling students to build strong fundamentals through structured self-study
• New coverage of monoclonal antibody production including upstream processing, downstream purification, quality control methods, and future industry perspectives
• Logical three-part organization progressing from basic concepts through process aspects to practical applications without requiring prior engineering background

Undergraduate students in biotechnology, chemical engineering, and biology will find this textbook builds essential bioprocess engineering competencies systematically. Lecturers at universities and applied sciences institutions gain a proven resource that connects theory to industrial practice across food, pharmaceutical, and water treatment applications.

Preface to the Third Edition xiii
Preface to the Second Edition xv
Preface to the First Edition xvii
Nomenclature xix
About the Companion Website xxiii

Part I Basic Concepts and Principles 1

1 Introduction 3
1.1 Background and Scope 3
1.2 Dimensions and Units 4
1.3 Intensive and Extensive Properties 6
1.4 Equilibria and Rates 6
1.5 Batch Versus Continuous Operation 7
1.6 Material Balance 8
1.7 Energy Balance 9

2 Elements of Physical Transfer Processes 13
2.1 Introduction 13
2.2 Heat Conduction and Molecular Diffusion 13
2.3 Fluid Flow and Momentum Transfer 15
2.4 Laminar Versus Turbulent Flow 17
2.5 Transfer Phenomena in Turbulent Flow 20
2.6 Film Coefficients of Heat and Mass Transfer 22

3 Chemical and Biochemical Kinetics 27
3.1 Introduction 27
3.2 Fundamental Reaction Kinetics 27

4 Cell Kinetics 45
4.1 Introduction 45
4.2 Cell Growth 45
4.3 Growth Phases in Batch Culture 47
4.4 Factors Affecting Rates of Cell Growth 49
4.5 Cell Growth in Batch Fermentors and Continuous Stirred-Tank Fermentors 51

Part II Unit Operations and Apparatus for Biosystems 55

5 Heat Transfer 57
5.1 Introduction 57
5.2 Overall Coefficients U and Film Coefficients h 57
5.3 Mean Temperature Difference 60
5.4 Estimation of Film Coefficients h 62
5.5 Estimation of Overall Coefficients U 66

6 Mass Transfer 71
6.1 Introduction 71
6.2 Overall Coefficients K and Film Coefficients k of Mass Transfer 71
6.3 Types of Mass Transfer Equipment 75
6.4 Models for Mass Transfer at the Interface 78
6.5 Liquid-Phase Mass Transfer with Chemical Reactions 79
6.6 Correlations for Film Coefficients of Mass Transfer 81
6.7 Performance of Packed Column 84

7 Bioreactors 93
7.1 Introduction 93
7.2 Some Fundamental Concepts 94
7.3 Bubbling Gas-Liquid Reactors 102
7.4 Mechanically Stirred Tanks 106
7.5 Gas Dispersion in Stirred Tanks 114
7.6 Bubble Columns 115
7.7 Airlift Reactors 119
7.8 Packed-Bed Reactors 121
7.9 Microreactors 122

8 Membrane Processes 127
8.1 Introduction 127
8.2 Dialysis 128
8.3 Ultrafiltration 129
8.4 Microfiltration 132
8.5 Reverse Osmosis 133
8.6 Membrane Modules 135

9 Cell-Liquid Separation and Cell Disruption 139
9.1 Introduction 139
9.2 Conventional Filtration 139
9.3 Microfiltration 141
9.4 Centrifugation 142
9.5 Cell Disruption 144

10 Sterilization 149
10.1 Introduction 149
10.2 Kinetics of Thermal Death of Cells 149
10.3 Batch Heat Sterilization of Culture Media 150
10.4 Continuous Heat Sterilization of Culture Media 151
10.5 Sterilizing Filtration 154

11 Adsorption and Chromatography 159
11.1 Introduction 159
11.2 Equilibria in Adsorption 159
11.3 Rates of Adsorption into Adsorbent Particles 161
11.4 Single- and Multistage Operations for Adsorption 162
11.5 Adsorption in Fixed Beds 163
11.6 Separation by Chromatography 167
11.7 Biorecognition Assay 175

Part III Practical Aspects in Bioengineering 181

12 Fermentor Engineering 183
12.1 Introduction 183
12.2 Stirrer Power Requirements for Non-Newtonian Liquids 185
12.3 Heat Transfer in Fermentors 186
12.4 Gas-Liquid Mass Transfer in Fermentors 189
12.5 Criteria for Scaling-Up Fermentors 195
12.6 Modes of Fermentor Operation 197
12.7 Fermentors for Animal Cell Culture 203

13 Instrumentation and Control of Bioprocesses 207
13.1 Introduction 207
13.2 Instrumentation of Bioprocesses 208
13.3 Control of Bioprocesses 213
13.4 Advanced Control of Bioprocesses 221

14 Downstream Operations