In October 1982, a small international symposium was held at the Gesellschaft fUr Strahlen- und Umweltforschung mbH (GSF) in Munich as a satellite meeting of the IX International Conference on Analytical Cytology. The symposium focussed on cytometric approaches to biological dosimetry, and was, to the best of our knowledge, the first meeting on this subject ever held. There was strong encouragement from the 75 attendees and from others to publish a proceedings of the symposium. Hence this book, containing 30 of the 36 presentations, has been assembled. Dosimetry, the accurate and systematic determination of doses, usually refers to grams of substance administered or rads of ionization or some such measure of exposure of a patient, a victim or an experimental system. The term also can be used to describe the quantity of an ultimate, active agent as delivered to the appropriate target material within a biological system. Thus, for mutagens, one can speak of DNA dosimetry, meaning the number of adducts produced in the DNA of target cells such as bone-mar row stem cells or spermatogonia.

Cytogenetic Effects

An Overview of Radiation Dosimetry by Conventional Cytogenetic Methods
Cytogenetic Effects in Human Lymphocytes as a Dosimetry System
Flow Cytometric Detection of Aberrant Chromosomes
Flow Cytometric Measurement of Cellular DNA Content Dispersion Induced by Mutagenic Treatment
Flow Cytometric Analysis of Chromosome Damage After Irradiation: Relation to Chromosome Aberrations and Cell Survival
Chromosome Analysis of Leukemia and Preleukemia: Preliminary Data on the Possible Role of Environmental Agents
Radiation Dosimetry Using the Methods of Flow Cytogenetics
Preliminary Reanalysis of Radiation-Induced Chromosome Aberrations in Relation to Past and Newly Revised Dose Estimates for Hiroshima and Nagasaki A-Bomb Survivors
Reproductive Effects
Biological Dosimetry of Sperm Analyzed by Conventional Methods: Cautions and Opportunities
Detection of Male Reproductive Abnormalities by Flow Cytometry Measurements of Testicular and Ejaculated Germ Cells
Cytometric Analysis of Mammalian Sperm for Induced Morphologic and DNA Content Errors
Mammalian Spermatogenesis as a Biological Dosimeter for Ionizing Radiation
Mutagenic Effects
Biological Dosimetry of Mutagenesis: Principles, Methods, and Cytometric Prospects
Detection of HGPRT-Variant Lymphocytes Using the FIP High-Speed Image Processor
Somatic Mutations Detected by Immunofluorescence and Flow Cytometry
Computer Scoring of Micronuclei in Human Lymphocytes
Hematopoietic and Immunologic Effects
Review of Biological Dosimetry by Conventional Methods in Haematopoiesis
Assessment of Environmental Insults on Lymphoid Cells as Detected by Computer Assisted Morphometric Techniques
Dose Related Changes in Cell Cycle of Bone Marrow and Spleen Cells Monitored by DNA/RNA FlowCytometry
Hoechst 33342 Dye Uptake as a Probe of Membrane Permeability in Mammalian Cells
Probing Macromolecular Structures by Flow Cytometric Fluorescence Polarization Measurements
Physiological and Preparatory Variation of Nuclear Chromatin as a Limiting Condition for Biological Dosimetry by Means of High Resolution Image Analysis
Morphologic, Kinetic, and Metabolic Effects
Automated Autoradiographic Analysis of Tumor Cell Colonies in vitro
Flow Cytometric Quantification of Radiation Responses of Murine Peritoneal Cells
Cell Cycle Kinetics of Synchronized EAT-Cells After Irradiation in Various Phases of the Cell Cycle Studied by DNA Distribution Analysis in Combination with Two-Parameter Flow Cytometry Using Acridine Orange
Nuclear Morphology of Hepatocytes in Rats After Application of Polychlorinated Biphenyls
Computer Graphic Displays for Microscopists Assisting in Evaluating Radiation-Damaged Cells
Flow Cytometric Measurement of Intracellular pH: A Dosimetry Approach to Metabolic Heterogeneity
Flow Cytometric Measurement of the Metabolism of Benzo [A] Pyrene by Mouse liver Cells in Culture
High-Affinity Monoclonal Antibodies Specific for Deoxynucleosides Structurally Modified by Alkylating Agents: Applications for Immunoanalysis.