A huge amount of fixed and paraffin-embedded tissue is stored in every hospital. This is very precious material that can be used for translational research and for diagnostics. The molecular methods employed for analysis of these tissues are similar to the usual molecular biology and proteomics methods, but reliable results can be obtained only if specific steps are followed with great care. This book provides detailed and precise guidelines for molecular analysis of archive tissues and will serve as an invaluable aid for researchers and pathologists involved in translational research and diagnostics. The topics addressed include pre-analytical treatment of tissues, isolation of tissue components, DNA and RNA methods, proteomics methods, internal quality control procedures, and storage and treatment of reagents. Clear notes and explanations are included to simplify use of the protocols for the less experienced. The authors are a group of acknowledged experts who have developed the described methods and validated them within the European project "Archive Tissues: Improving Molecular Medicine Research and Clinical Practice - IMPACTS", which has involved 21 leading institutions in 11 countries.

Introduction

Archive Tissues
Pre-Analytical Treatment of Tissues
Dissection of Tissue Components
Extraction, Purification, Quantification and Quality Assessment of Nucleic Acids
Reverse Transcription (RT)
Nucleic Acid Amplification
DNA Sequencing
Microsatellite Analysis
Methylation Analysis
Analysis of Copy Number Variations
Microarrays
Protein Extraction in Archive Tissues
Protein Electrophoretic Separation
Protein Immunodetection
Mass Spectrometry
Elements of Good Laboratory Practice