The timely volume describes recent discoveries and method developments that have revolutionized Structural Biology with the advent of X-ray Free Electron Lasers. It provides, for the first time, a comprehensive examination of this cutting-edge technology. It discusses of-the-moment topics such as growth and detection of nanocrystals, Sample Delivery Techniques for serial femtosecond crystallography, data collection methods at XFELs, and more. This book aims to provide the readers with an overview of the new methods that have been recently developed as well as a prospective on new methods under development. It highlights the most important and novel Structural Discoveries made recently with XFELS, contextualized with a big-picture discussion of future developments.

X-ray Free Electron Lasers and their applications

Serial Femtosecond Crystallography (SFX): An Overview
Small is Beautiful: Growth and Detection of Nanocrystals
The lipid cubic phase as a medium for the growth of membrane protein microcrystals
Sample Delivery Techniques for Serial Crystallography
When diffraction stops and destruction begins
Climbing the data mountain: processing of SFX data
Phasing XFEL serial crystallography data
Structure Determination by Continuous Diffraction from Imperfect Crystals
Advances in structure determination of G protein-coupled receptors by SFX
Time-resolved serial femtosecond crystallography, towards molecular movies of biomolecules in action
Towards Molecular Movies of Enzymes
X-ray Spectroscopy with XFELs
Single molecule imaging using X-ray free electron lasers
The use of angular correlations to extract 3-dimensional structural information from X-ray solution scattering
Future Directions of High Repetition Rate X-rayFree Electron Lasers
Index.