This book describes the methods of analysis and determination of oxidants and oxidative stress in biological systems. Reviews and protocols on select methods of analysis of ROS, RNS, oxygen, redox status, and oxidative stress in biological systems are described in detail. It is an essential resource for both novices and experts in the field of oxidant and oxidative stress biology.

Introduction.- Assays for thiols and modifications

Pitfalls of ROS measurements by fluorescent probes and mitochondrial superoxide determination using MitoSOX
Methods and Reagents.- Clinical Probes for ROS and Oxidative Stress
Measurement of oxidative stress markers in vitro using commercially available kits
Oxidative Lipidomics - Analysis of Oxidized Lipids and Lipid Peroxidation in Biological Systems with Relevance to Health & Disease
Clinically Related Models and Approaches
Oxidant-Induced Models of Vascular Leak
Ozone-Specific' Oxysterols and Neuronal Cell Signaling
Measurement Oxidative Stress Status in Human Populations: A Critical Need for a Metabolomic Approach
Instrumental Methods
Sense and Sensibility of Oxygen in Pathophysiology using EPR Oximetry
Resonators for Clinical EPR
Biomedical Overhauser Magnetic Resonance Imaging (OMRI).  Noninvasive Imaging of Redox Processes
Index.