1;Preface;5 2;List of Contents;8 3;List of Contributors;10 4;Tools for Conditional Mutagenesis;11 4.1;Conditional Somatic Mutagenesis in the Mouse Using Site- Specific Recombinases;12 4.2;Cre/loxP-Mediated Chromosome Engineering of the Mouse Genome;38 4.3;Tetracycline-Controlled Genetic Switches;58 4.4;Novel Gene Switches;82 4.5;Improved Embryonic Stem Cell Technologies;115 4.6;Gene Trap Mutagenesis;137 4.7;RNA Interference in Mice;156 4.8;Viral Vectors: AWide Range of Choices and High Levels of Service;184 4.9;Conditional Mutagenesis by Cell-Permeable Proteins: Potential, Limitations and Prospects;210 5;Examples of Conditional Disease Models;240 5.1;Analysis of Mouse Development with Conditional Mutagenesis;241 5.2;Conditional Mouse Models of Cancer;269 5.3;Conditional Mutagenesis Reveals Immunological Functions ofWidely Expressed Genes: Activation Thresholds, Homeostatic Mechanisms and Disease Models;294 5.4;Conditional Transgenesis and Recombination to Study the Molecular Mechanisms of Brain Plasticity and Memory;320 5.5;A Novel Conditional Knockout Strategy Applied to Serotonin Receptors;351 5.6;Conditional Mouse Models for Friedreich Ataxia, a Neurodegenerative Disorder Associating Cardiomyopathy;368 5.7;Animal Models in Cardiovascular Diseases: New Insights from Conditional Models;379 5.8;Conditional Animal Models for the Study of Lipid Metabolism and Lipid Disorders;408 5.9;Conditional Mouse Models to Study Developmental and Pathophysiological Gene Function in Muscle;441 5.10;Analysis of Calcium Channels by Conditional Mutagenesis;469 5.11;Conditional Mutagenesis of G-Protein Coupled Receptors and G- Proteins;491 5.12;Contribution of Targeted Conditional Somatic Mutagenesis to Deciphering Retinoid X Receptor Functions and to Generating Mouse Models of Human Diseases;510 6;Subject Index;524