1;About the Book;6 2;About the Editor;8 3;Preface;10 4;Contents;12 5;List of Contributors;14 6;Part I Introduction;18 6.1;Chapter 1:An Overview of HPTLC: A Modern Analytical Technique with Excellent Potentialfor Automation, Optimization, Hyphenation,and Multidimensional Applications;19 6.1.1;Journey of Thin-Layer Chromatography;22 6.1.2;Recent Developments;23 6.1.2.1;Over-Pressured Layer Chromatography;23 6.1.2.2;Planar Electrochromatography;23 6.1.2.3;Image Analysis;24 6.1.2.4;Two-Dimensional Separations;25 6.1.3;High-Performance Thin-Layer Chromatography (HPTLC);25 6.1.3.1;HPTLC: Separation and Resolution;27 6.1.3.2;HPTLC: Validation Process;28 6.1.3.2.1;Selectivity;28 6.1.3.2.2;Sensitivity;29 6.1.3.2.3;Precision;29 6.1.3.2.4;Accuracy;29 6.1.3.2.5;Ruggedness;29 6.1.3.2.6;Limit of Detection;30 6.1.3.2.7;Stability;30 6.1.3.3;HPTLC: Optimization and Process Control;30 6.1.3.4;HPTLC: Automation;31 6.1.3.5;HPTLC: Hyphenation;32 6.1.4;Liquid Chromatography-Thin-Layer Chromatography (LC-TLC);33 6.1.5;High-Performance Thin-Layer Chromatography-Mass Spectrometry (HPTLC-MS);33 6.1.6;High-Performance Thin-Layer Chromatography-Infrared Spectroscopy (HPTLC-IR);34 6.1.6.1;HPTLC: Laser;35 6.1.6.2;HPTLC: Multidimensional Applications;36 6.1.7;Notes;36 6.1.8;Suggested Readings;37 7;Part II Fundamentals, Principle and Advantages of HPTLC;41 7.1;Chapter 2: Fundamentals and Theory of HPTLC-Based Separation;42 7.1.1;Planar Chromatography;43 7.1.2;Theoretical Considerations;43 7.1.2.1;Separation Efficiency;43 7.1.2.2;Partition Coefficient;45 7.1.2.3;Retention/Retardation Factor;46 7.1.2.4;Capacity Factor;48 7.1.2.5;Spot Capacity;49 7.1.2.6;Plate Height;49 7.1.2.7;Resolution;51 7.1.2.8;Selectivity;52 7.1.3;Notes;53 7.1.4;References;53 7.2;Chapter 3: Experimental Aspects and Implementation of HPTLC;55 7.2.1;HPTLC Methodology;56 7.2.1.1;Stationary Phase;59 7.2.1.2;Layer Prewashing;59 7.2.1.3;Mobile Phase;60 7.2.1.4;Sample Preparation and Application;60 7.2.1.5;Development of Chromatogram;61 7.2.1.5.1;Detection;61 7.2.1.5.2;Derivatization;62 7.2.1.5.3;Immersing;62 7.2.1.5.4;Spraying;62 7.2.1.5.5;Heating;62 7.2.1.5.6;Quantification;62 7.2.1.5.7;Documentation;63 7.2.2;Validation of Method;63 7.2.2.1;Specificity;64 7.2.2.2;Sensitivity;64 7.2.2.3;Linearity and Calibration Curve;64 7.2.2.4;Accuracy;65 7.2.2.5;Precision;65 7.2.2.6;Repeatability;65 7.2.2.7;Robustness;65 7.2.2.8;Retardation Factor;65 7.2.2.9;Peak Purity;66 7.2.3;Notes;67 7.2.4;References;67 7.3;Chapter 4: High-Performance Thin-Layer Chromatography: Excellent Automation;69 7.3.1;Automation of TLC to HPTLC;69 7.3.2;Protocol;71 7.3.2.1;Preparation of Plate;71 7.3.2.2;Plate Size;71 7.3.2.3;Sample Application Position;71 7.3.2.4;Chromatogram Development;72 7.3.2.5;Derivatization;72 7.3.2.6;Image Documentation;72 7.3.3;Auto Spray Loading;72 7.3.4;Automated Development;73 7.3.5;Digital Camera-Based Image Documentation;76 7.3.6;Software-Induced Scanning;76 7.3.7;Notes;79 7.3.8;References;79 8;Part III Applications of HPTLC Separation;80 8.1;Chapter 5: Multidimensional and Multimodal Separations by HPTLC in Phytochemistry;81 8.1.1;Multiple Development Techniques;83 8.1.1.1;Unidimensional Multiple Development;85 8.1.1.2;Incremental Multiple Development;87 8.1.1.3;Gradient Multiple Development;88 8.1.1.4;Bivariant Multiple Development and its Automated Version: Automated Multiple Development;91 8.1.2;Multidimensional Thin-Layer Chromatography;92 8.1.3;Comprehensive 2D Chromatography on One Adsorbent;92 8.1.3.1;Two-Dimensional Separations on Polar Adsorbents: Silica, Cellulose, Polyamide;93 8.1.3.2;Two-Dimensional Separations on Polar Chemically-Bonded Stationary Phases: CN-Silica, DIOL-Silica, and NH2-Silica;95 8.1.4;2D-TLC on Bilayer Plates and Coupled-Layer 2D-TLC;96 8.1.5;Combination of MD-PC Methods: Hyphenated Techniques;99 8.1.6;Notes;101 8.1.7;References;101 8.2;Chapter 6: Stability-Indicating HPTLC Determination of Imatinib Mesylate in Bulk Drug and Pharmaceutical Dosage;105 8.2.1;Method Valida