This textbook provides an accessible and comprehensive introduction to the basic principles of immunological methods. It offers a fundamental insight into the performance of different immunoassays and their areas of application.

Each chapter in Immunoassays begins with a basic introduction to the topic before going on to cover important details for everyday laboratory work and professional practice. Readers will gain confidence in using the methods as potential problems are discussed in detail and troubleshooting sections help to solve them. The book covers all the classic and essential techniques, such as enzyme-linked immunosorbent assay (ELISA), dot blot, and western blot, as well as the latest advances in polymerase chain reaction (PCR), flow cytometry, fluorescence microscopy, and 3D cell and organoid culture. Importantly, the book also covers regulatory requirements and provides information on quality systems with various mandatory guidelines and regulations, such as in-vitro diagnostics, medical devices, the pharmaceutical industry, and food analysis.

Packed with many practical tips, this textbook is an invaluable resource for beginners and experienced practitioners alike. It is aimed at researchers and students in the life sciences and biomedicine, as well as laboratory assistants.

Forewords

Introduction to Immunoassays
Antibodies
ELISA/EIA/FIA
Sample Preparation for Immunoassays
Lateral Flow Immunoassays
Immuno-PCR: Application-oriented highly sensitive protein analytics with antibody-DNA conjugates
Evolution of PCR from classical PCR to digital PCR
Detection of Proteins on Membranes: Western Blot and Dot Blot Methods
Acceleration of Western Blotting
Lab-on-a-Chip Miniaturization of Assays for On-Site Diagnostic Applications Using Microfluidics
Reliable protein quantification by surface plasmon resonance spectroscopy
Troubleshooting and Assistance in the Development, Validation and Implementation of ELISAs
Detection Methods for Protein Interaction
Immunohistochemistry for Research and Diagnostic Pathology
Fluorescence Microscopy in Immunoassay Application An Introduction
In Vivo Immunostaining and Intravital Two-Photon Microscopy
Quantitative digital holographic phase contrast microscopy biophysical characterization of immunological processes, cells and tissues
Circulating Tumor Cells Isolation and Molecular Characterization
Automated isolation of circulating tumor cells for liquid biopsy
3D Cell and Organoid Culture
Tissue Engineering and Regenerative Medicine
The CRISPR/Cas Technology: Classic and Future Applications in Molecular Biology and Medicine
Fluorescent Dyes
Stabilization of Assay Components
Interference Effects in Immunoassays
Troubleshooting and Assistance in the Development, Validation and Implementation of ELISAs
Evaluation and Validation
Food and Feed Analysis
Automation of Immunoassays
Challenges for a Next Generation Biobanking Cryopreservation for the advancement of personalized medicine
The new EU Regulation 2017/746 on in vitro diagnostic medical devices
ISO 13485 the mandatory quality system for IVD manufacturers
Clinical Trials for In-vitro Diagnostics
Protection for Diagnostic Methods and its Limits under the European Patent Convention and the German Patent Law
Learning Laboratory Systems: How can artificial intelligence support laboratory work?
Impedance-based Analysis of Functional Immunoassays using Cultured Cells
Measurement of Single Molecules and Cells with Atomic Force Microscopy
Immunoassays for Extracellular Vesicles-based Characterization
Immunoassay Fabrication and Device Functionalization by Scanning Probe Lithography
The digital lab of the future, laboratory information systems, analytics and AI in LifeScience R&D
Regulation of Medical Devices in the USA with a Focus on IVDs
Optical Coherence Tomography for Non-Invasive Imaging of Tissue and Organoids
sFIDA: Quantitative Single Particle Analysis
The Blood-Brain Barrier
The Biomedical Lab, Ethics, and Obtaining Ethics Approval for Research
A label-free future: digid´s BioMEMS platform enables diagnostics beyond the laboratory
Ethical Boundaries of Research Freedom
New Approaches in Personalized Tumor Therapy Research: Organoids, Organoid-on-Chip, and Tumor Explants as Models.