Throughout our journey in infection control practice, training & teaching, the claim for a handy guide became a necessity. This book offers a simplified approach for infection control application targeting junior health care professionals. It highlights the fundamentals, so they could adopt their role in health care system putting the safety as a first priority (FIRST DO NO HARM). The book includes 16 chapters covering the updates in basic infection control practices starting by host parasite relationship, common types of hospital acquired infections, infection chain, standard & transmission based precautions. The book ends by a summary of applied measures in critical hospital wards (ICU, NICU& OR) and care bundles that address the evidenced based practices. The 1st edition was published in 2014. It was greatly appreciated from readers. The book is prepared by a group of associate professors & lecturers of Medical Microbiology & Immunology, Zagazig University, Egypt : Rehab El-Sokkary, Rehab Tash, Noha Hammad, Mai Malek, Alia El-Shahaway, Randa Saddek, Enas Tantawy, Manar Soliman, Raghdaa Ramadan, Rania Aamer, Wafaa Metwally, Manar Gebriel, Lobna El-Korashi, Noura Metwaly.