Destined to set the standard, the first book dealing exclusively with this revolutionary and novel imaging technology serves as an easy-to- understand introduction while offering numerous tips and tricks.
Adopting a practical approach, the authors who developed this actual technique provide a comprehensive, hands-on overview of the basics of light sheet fluorescence microscopy, instrumentation, applications, sample preparation, and data analysis. As a reflection of the uncompromisingly interdisciplinary nature of the topic, they merge their expertise in physics, biology, and computer science, giving valuable insider tips taken from their work at major manufacturers.
The result is in-depth information on hardware and software solutions for a straightforward implementation of LSFM in the lab.

LIGHT SHEET MICROSCOPY HARDWARE<br>Physics of Light Sheet-based Fluorescence Microscopes (e.g. Light Sheet formation, Optics, Detectors)<br>Overview of the Flavors of Light Sheet Microscopy (e.g. Arrangements of Optical Axes, Schematics, Commercial Set-ups, Extensions to Other Modalities)<br>Driving the Microscope and Open Access Electronics (e.g. Software to Pilot Light Sheet Microscopes (MicroManager), Arduino Solutions)<br>"Do It Yourself" Light Sheet based Microscope (e.g. OpenSPIM/OpenSPIN/LegoSPIM)<br> <br>SAMPLE MOUNTING FOR LIGHT SHEET MICRSOCOPY<br>Sample Handling and Preparation (Including Sample Chamber Designs, Sample Positioning)<br>Clearing of Biological Tissues For Light Sheet Microscopy<br>Operation of Light Sheet-based Fluorescence Microscopes (Including In Multi-user Environment)<br> <br>LIGHT SHEET MICRSOCOPY SOFTWARE<br>Image Processing of Multi-view Data (Registration, Fusion, and Deconvolution))<br>Visualization of Big Image Data (e.g. Local and Remote Viewing, 3D Rendering, Annotation)<br>Information Technology of Big Data Handling (e.g. Compression, Storage, Transfer)<br>Extracting Information From Light Sheet Datasets (e.g. Image Analysis Such As Atlas Registration, Segmentation, Tracking)<br> <br>LIGHT SHEET MICROSCOPY APPLICATIONS<br>Applications In Developmental Biology<br>Applications In Cell Biology<br>Applications In Neurobiology<br>Applications In Biodiversity<br> <br>LIGHT SHEET MICROSCOPY IMPLEMENTATIONS<br>Imaging Platform Integration<br>Laboratory Integration<br>IT Supports